Seyed Javad Mowla, Ensieh Poursani, Majid Mehravar, James E. Trosko
Alternative splicing is an important mechanism that regulates gene expression and function in mammals, particularly in primates and human. Large-scale studies estimates a large number of human genes undergo alternative splicing. OCT4, a crucial stem cell marker, is one of the most important stemness markers that is not excluded in this regard. Up until now, it is known that OCT4 can produce several spliced variants in different cell types. In this study, we used RT-PCR and DNA sequencing approaches to verify OCT4 variant expression. Here, we reported a novel OCT4 spliced variant which is named as OCT4B3, for the first time. This variant is very similar to the OCT4B2 transcript, but a 207-bp fragment has been lost from Exon1b. Moreover, we investigated the expression pattern of OCT4B3 in several human cancer cell lines and found its expression in two cell lines of 1321N1 and 5637. Our experiments resulted in discovery of a novel OCT4 variant of OCT4B3 that is expressed in two human 5637 and 1321N1 cell lines.
Sadaf Iqbal, Afeefa Kiran Chaudhary, Mahwish Anwar, Hassan Akbar Khan, IIftikhar Ejaz, Qazi Muhammad Tauseef, Ayesha Rehman, Mahmood Hassan Qazi
Pakistan is one of the developing countries that had been severely infected with Dengue virus in past years. In order to assist the clinicians, studies were required to characterise the extent and type of dengue virus infection and this lapse result in the huge loss of lives within 3 years. This study elucidated the infection status of children with fever at various day of presentation utilising PCR based qualitative and genotypic assays. A total of 83 children were evaluated, out of which only ten were found to be PCR positive. This detection was dependent on antibody and antigen status of the patient using RT-PCR. Moreover, all the PCR positive patients were found to be infected with serotype 2 (DENV-2). All of these subjects belonged to the group of children that were sampled in first five days of their fever. It is therefore concluded that conventional PCR may serve as an efficient tool for diagnosis of dengue but at a very earlier stage of the disease. Further studies are needed to provide a better screening of the prevalent type of serotypes.