Research Article

Overexpression of Chemokine Receptors on Neural Stem Cells Pretreated with Valproic acid: Towards Improved Homing

Fahime Karimi, Mohammad Reza Hashemzadeh, Mohammad Amin Edalatmanesh , Hojjat Naderi-Meshkin

Fahime Karimi
Iranian Academic Center for Education, Culture Research (ACECR), Khorasan Razavi Branch

Mohammad Reza Hashemzadeh
Royesh Stem Cell Biotechnology Institute, Mashhad

Mohammad Amin Edalatmanesh
Islamic Azad University, Shiraz Branch. Email: amin.edalatmanesh@gmail.com

Hojjat Naderi-Meshkin
Iranian Academic Center for Education, Culture Research (ACECR), Khorasan Razavi Branch
Online First: April 20, 2018 | Cite this Article
Karimi, F., Hashemzadeh, M., Edalatmanesh, M., Naderi-Meshkin, H. 2018. Overexpression of Chemokine Receptors on Neural Stem Cells Pretreated with Valproic acid: Towards Improved Homing. Journal of Genes and Cells 4: 33-39. DOI:10.15562/gnc.64


Neural stem cells (NSCs) have considerable capacity for self-renewing and also ability for generating neurons in the mammalian brain. However, one of the big challenges is the migration and targeted homing of transplanted NSCs into the injured site to treat neurodegenerative diseases including Alzheimer´s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), brain ischemia (BI) and spinal cord injury (SCI). To improve homing capacity, pretreatment of NSCs with Valproic acid (VPA), which is supposed to cause diverse effects on migration ability of NSCs, is a strategy. More recently, hind brain and olfactory bulbs have been introduced as a good source of NSCs. So, NSCs were isolated from these two sources of postnatal day 1 (PND1) rats. These isolated cells were characterized by expressing neuronal markers such as Nestin and Sox2. The expression of four selected chemokine receptors (CXCR4, CXCR6, CCR1 and CCR7), which are important effectors in homing of stem cells, was investigated. It is concluded that VPA treatment enhances NSCs migration and homing showing its potential to be applied for cell-based therapies.

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