Short Communication

Discovery of a Novel Variant of OCT4, OCT4B3

Seyed Javad Mowla , Ensieh Poursani, Majid Mehravar, James E. Trosko

Seyed Javad Mowla
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Email: sjmowla2@gmail.com

Ensieh Poursani
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Majid Mehravar
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

James E. Trosko
Institute of Integrative Toxicology, Michigan State University
Online First: October 01, 2016 | Cite this Article
Mowla, S., Poursani, E., Mehravar, M., Trosko, J. 2016. Discovery of a Novel Variant of OCT4, OCT4B3. Journal of Genes and Cells 2(3-4): 32-35. DOI:10.15562/gnc.38


Alternative splicing is an important mechanism that regulates gene expression and function in mammals, particularly in primates and human. Large-scale studies estimates a large number of human genes undergo alternative splicing. OCT4, a crucial stem cell marker, is one of the most important stemness markers that is not excluded in this regard. Up until now, it is known that OCT4 can produce several spliced variants in different cell types. In this study, we used RT-PCR and DNA sequencing approaches to verify OCT4 variant expression. Here, we reported a novel OCT4 spliced variant which is named as OCT4B3, for the first time. This variant is very similar to the OCT4B2 transcript, but a 207-bp fragment has been lost from Exon1b. Moreover, we investigated the expression pattern of OCT4B3 in several human cancer cell lines and found its expression in two cell lines of 1321N1 and 5637. Our experiments resulted in discovery of a novel OCT4 variant of OCT4B3 that is expressed in two human 5637 and 1321N1 cell lines.

No Supplementary Material available for this article.
Article Views      : 1396
PDF Downloads : 565